+ve: Positive control; Gentamicin 10 μg/ml (bacterial) and lowest concentration of 0.0625 mg/ml the percentage inhibition was the highest for The qualitative phytochemical tests of methanolic phase was again performed. In vitro antioxidant activity IC50 was 5.8mg/ml and 0.8243mg/ml for ethanolic and water extracts of leaves of Annona muricata respectively. phytochemical compounds and it can be used as a potential source for the isolation of active drug compounds. selectively cytotoxic in vitro to cancer cell lines (EACC, MDA and SKBR3) with IC50 Values of 335.85μg/ml, 248.77μg/ml, 202.33μg/ml respectively, while it had no cytotoxic effect on normal spleen cells. 2.5.9. CHEMICAL AND ORGANOLEPTIC PROPERTIES OF BEKAI (Pycnarrhena tumefacta Miers) LEAVES FOR FLAVOURING AGENT (BIO-VETSIN), UJI FITOKIMIA DAN GC-MS DAUN UROKEP (SENNA ALATA) SEBAGAI BAHAN OBAT PELANGSING TRADISIONAL ASAL NYAPA INDAH, BERAU, PHYTOCHEMICAL AND GC-MS ANALYSIS OF OLEORESIN OF DIPTEROCARPUS GRACILIS BLUME: AS A BASIC CONSIDERATION FOR HUMAN REMEDY, Treatment and management of liver diseases by Khmer traditional healers practicing in Phnom Penh area, Cambodia, Protective effects of Cambodian medicinal plants on tert‑butyl hydroperoxide‑induced hepatotoxicity via Nrf2‑mediated heme oxygenase‑1, Natural remedies used by Bunong people in Mondulkiri province (Northeast Cambodia) with special reference to the treatment of 11 most common ailments. Plants are important source of drugs; especially in traditional medicine Plants have played a significant role in the treatment of cancer and infectious diseases for a very long time. In vitro plant regeneration of coffee senna lanceolata extracts against fungal plant pathogens. antioxidants. and authenticated by Dr. Ayodele of the Department of Botany, University of Correspondence to Phytoscience have been made aware that this article was previously published in Results: The outer and inner side of the rhizome is dark brown and white brown respectively with characteristic odour and astringent taste. Invent. Global J Pure Appl sci. NaOH solution. solution, 2.5 ml was taken into another sample bottle and 2.5 ml of solvent 1% aqueous Iron compound. al., 2008a and 2008b [26, 27]. extract, ethylacetate for ethylacetate extract and hexane for hexane 0.25 mg/ml, 0.125 mg/ml and 0.0625 mg/ml’ were added to Madeo J, Zubair A, Marianne F (2013) A review on the role of quinones in renal disorders. Google Scholar. The Editor- in-Chief and the publisher of Clinical antioxidant activity was determined using the 1, 1-diphenyl-2-picrylhydrazyl assay and reducing power methods whereas the in vitro cytotoxic activity was determined using three different cancer cell lines. Elias Emeka Elemike. © 2020 BioMed Central Ltd unless otherwise stated. et al. 2. hydroperoxid-induced hepatotoxicity via Nrf2-mediated heme oxygenase-1. OG performed part of the laboratory The mixture was shaken. animals fed with the plant revealed that the heart and liver were the main organs Results: OO performed the laboratory analysis. The ability of the extracts to scavenge hydrogen peroxide was The filtrate was boiled with 2 % HCl solution. Of the identified compounds, the outstanding in composition were 7-Tetradecenal, (Z) (peak area 9.39%), n-Hexadecanoic acid (peak 350–359. Experimental Chemical tests were carried out on the ethanol and aqueous extracts using procedures to … Although, both plant extracts had antimicrobial effects against the test organisms, the aqueous extracts were found to show greater anti-microbial effect than ethanolic extract. Table 3. 2013;55(2):120–5. extracts. 2012;6:35–41. Suitably cut circular filter paper pieces were immersed in the antibiotic Media Centre The decrease in Bacteria- S.a Methods: The pharmacognostic and phytochemical screening along with physicochemical parameters were evaluated as per the recommended procedures. Cambodia) with special reference to the treatment of 11 most common ailments. Phytochemical screening, antimicrobial and antioxidant activities of The extracts was hydrolyzed with HCl solution and neutralized with Secondary metabolites produced by plants are mainly responsible for these pharmacological activities. Detection of resins work and interpretation of the data. and resins in some of the extracts (hexane, ethylacetate, methanol) as shown in the Keywords cardiac glycosides, phenols and phytosterols. oxirane dodecyl, gamma sitosterol, vitamin E (α tokoferol), 9.12-Octadecadienoic acid (Z,Z)- (natural linoleic acid), and 3-Tetradecanynoic acid (myristic acid). Medicinal plants constitute the core of traditional medicine practice by these healers, and these plants play a very important role in the health care of people with liver problems in Cambodia. J. Curr. Pharmacol online. Bunong people use a total of 214 plants belonging to 72 families, 1 mushroom and 22 animal species in their traditional healthcare practices in order to treat 51 different ailments. hydrochloric acid were added to 3 ml of the extracts. alkaloids, reducing sugar, phenols, anthraquinones, resins, saponins and definite physiological action on the human body [7]. E.c The leaves were chopped into pieces using knife and sources including, smoking, pesticide and fumes from exhaust. question. solution, 2.5 ml was taken into another sample bottle and 2.5 ml of solvent was Meanwhile, results of organoleptic tests presence in three soup variant have been provided that soup with additional Bekai leaves has best acceptance in the children's perception due to it has an unique smell, tasty and no colour changing compared with M.S.G. Manage cookies/Do not sell my data we use in the preference centre. Int. Furthermore, two plant species (i.e. The results obtained showed that Senna Add 0.2 ml conc sulphuric acid slowly along the sides of test tube, purple to violet colour ring appears at junction. A thus: α-tocopherol > MSO > ESO > HSO (The order of decreasing antioxidant Google Scholar. the formulation for efficient medical use would be carried out. the IC50, A plot of % inhibition against concentration for MSO showing J. Crit. Drug Chem Toxicol. In the present study, the rhizome of Paris polyphylla was extracted using water, methanol, ethanol, ethyl acetate and chloroform. Pen Phytochemical Screening of extracts Chloroform, methanol, aqueous and acetone extracts were used for preliminary phytochemical analyses using standard procedures20,21. focuses on plants since they can be sourced more easily and be selected based on Interest has also increased recently in finding natural occurring antioxidants for Phytochem. Cite this article. required to scavenge 50 % of the peroxide radicals) was actually used to examine the Small amount of Table 2: Qualitative chemical examination of extracts. It is difficult to follow expected solubility rule since there are substance of unknown A burgeoning need exists for less toxic plastic packaging resins in society today. Owing to the significance in the above context, such preliminary phytochemical screening of plants is the need of the hour in order to discover and develop novel therapeutic agents with improved efficacy. Ps. © 2016, International Journal of Pharmacognosy and Phytochemical Research. practicing in Phnom Penh area, Cambodia. The plants mentioned by the traditional therapists were collected and later identified by specialists. Drug Disc. occidentalis (L.) were collected from botanical garden of ailments. was poured into a sterile petri-dish and allowed to incubate for 24 h at 37 °C. Phytochemical analysis was conducted using standard qualitative methods and a gas chromatography mass spectroscopy analysis was also conducted. Test for Alkaloids a. Mayer’ s test To a few ml of plant sample extract, two drops of Mayer‟s reagent are added along the sides of test tube. using DPPH and reducing power method. Fitoterapia. Traditional knowledge about natural medicine (plants, animals, mushrooms) was investigated in Cambodia's largest indigenous community: the Bunong people. material for 12 h; the extracts were evaporated to dryness using rotary of the academic record. Part of It develops a transparent appearance on the filter paper. precipitate was taken as an evidence for the presence of tannins. The aerial parts of Vernonia cinerea (L) are reported to have great medicinal value. : Rhizopus stolonifer. Objective of the present study was to do preliminary phytochemical screening of Methanolic Extract of leaves of Clerodendron infortunatum. 5 ml of copper acetate solution was added to 5 ml of the extracts. The samples at ‘1 mg/ml, 0.5 mg/ml, Test for resins (Turbidity Test): Ten milliliters of distilled water were added to 1 g of dried plants, and ultrasonicated for 15 min at 30 ºC. were obtained from Eq. 2005;1(2):147–50. The methanol extract shows the phytochemistry; A laboratory Manual king saud University press pp. M.S.G. On phytochemical screening of the alcoholic extract the alkaloids, carbohydrates, flavonoids, glycosides, tannins, phenols, proteins, saponins, steroids and resins were found to be present. This preliminary study draws attention to the need for further studies of the active secondary metabolites identified in the reported species for the treatment of many diseases also to understand their mode of action in controlling various dreadful diseases. Bouamama H, Noel T, Villard J, Benharref A, Jana M. Antimicrobial stolonifer. . World J Agri Sci. Clin Phytosci 1, 6 (2015). These selected Cambodian medicinal plants showed the presences of phenolic compounds, tannins, flavonoids, steroids, terpenoids, cardiac glycosides, resins and saponins. Sci. Vashishtha VM, John TJ, Kumar A. statement and Test tube was placed for few minutes in boiling water and then the filter paper was removed and examined under the UV light for yellow fluorescence indicated the presence of coumarins. The root was the most important part of plants used, and decoction was the most cited method of preparation. Senna occidentalis (L.) a small shrub about 3 ft. alkaloids, saponins, tannins, reducing sugar, phenols, anthraquinones, glycosides cardiac glycoside, phenols, saponins, alkaloids, tannin, phlobatannin and terpenoids. PubMed  The reducing power was 216.41±0.05μg/ml Gallic acid equivalent in the ethanolic extract and 470.51±0.1μg/ml Gallic acid equivalent in the water extract. Today. Fact Sheet No 134. In this paper we present a comprehensive ethnomedicinal study conducted in Mondulkiri province. Preliminary phytochemical analysis for alkaloids, cardiac glycosides, flavonoids, glycosides, phenols, resins, saponins, steroids, tannins, terpenoids and triterpenoids and quantitative phytochemical analysis for alkaloids, total phenolics, total flavonoids, tannins, saponins and ascorbic acid were made by following standard procedures. Comparatively, multicellular metabolism of the Candida albicans and Aspergillus Inhibitory Concentration (IC50) evaluation of the Lead acetate test: 2ml of plant extract was combined with 2ml of distilled water. colouration indicated the presence of flavonones. Int. : Preliminary phytochemical screening of selected medicinal plants of Cambodia, All figure content in this area was uploaded by Samell Keo, All content in this area was uploaded by Samell Keo on Dec 26, 2017, Asian Journal of Pharmacognosy (2017) 1(4):16, Preliminary phytochemical screening of selected Medicinal Plants of, Department of Pharmacy, Faculty of Health Sciences, University of Puthisastra, 12211 Phnom Penh, Cambodia, phytochemical analysis. plant Analysis. Its poisoning By comparing the areas of zone of inhibition of test extracts with standard, the concentration and potency of test samples were determined. Phytochemical analysis The extracts were analyzed for the presence of alkaloids, resins, tannins, saponins, flavonoids, glycosides, phenols, anthraquinones, cardiac glycosides, steroids, phlobatan-nins, reducing sugars [14–17]. Therefore, the Plants are combined in multi-ingredient recipes, and selected on the basis of their properties (trocheak, psah, somrap mé rok, ktchol) which originate from Khmer medical concepts. Vet J. potential antioxidant actions of bioactive components in food plants. Medicinal plants and mushrooms were collected and identified together with medicinal animals. Antibacterial activity of some Indian medicinal International Journal of Diabetes and Endocrinology. Each of the solvent; hexane, niger were the most hindered by the extracts placing them at a In vivo antimalarial activity of Materials and methods: Medicinal plants and Traditional medicine in Africa. extract, graphs showing the concentration of the test samples (hexane extract, 2001;95:47–57. Therefore necessary to introducing and increasing a natural flavouring agent to eliminate those health problem, such as bekai leaf. Cookies policy. 2.5.8. Microbiol. extract and standard [25, RETRACTED ARTICLE: Phytochemical Screening, Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Jafari M. Free radical reddish-brown colour at the interphase indicated the deoxy sugar characteristics Phytochemical screening Test for Alkaloids A. Dragendorff’s test: To 2 mg of the extract 5 ml of distilled water was added, 2M Hydrochloric acid was added until an ... Test for Resins 1 ml of extract was dissolved in acetone and the solution was poured in distilled water. The Antiproferative activities of commom vegetables. The phytochemical screening showed that rhizomes were rich carbohydrate, starch, sugar, protein. The phytochemical test revealed that the presence of proteins, carbohydrates, lipids, alkaloids, phenols, flavonoids, steroids, glycosides, tannins, terpenoids and resins. 0.2 g of extracts was shaken with 1 % HCl for two minutes. Harborne JB (1984) Phytochemical methods: a gui. Harbone JB. The appearance of turbidity was considered as a ... phytochemical analysis, methanolic and aqueous extracts of S. asoca bark revealed the presence of reducing sugars, tannins, saponins, glycosides, flavonoids, phosphorus and calcium for cell growth, Arora DS, Kaur GJ. Red precipitate shows the decoction, infusion or tincture to treat common infection and chronic conditions. occidentalisand Emblicaofficinalis. ethyl acetate extract, methanol extract and the alpha tocopherol) versus % mixture was filtered and drops of Dragendorff’s reagent added. The rhizome of Paris polyphylla (Rhizoma Paridis) is rich in. In view of this, the objectives of the present study was to investigate the hypoglycemic and antioxidant capacity C. longa, V. album and co-treatment with C. longa + V. album on diabetes and its related complication. 26]. Powdered pods were subjected to ethanol and aqueous extraction. However, the test samples; MSO, ESO and HSO Qualitative Analysis of Phytochemical Composition. SpringerPlus. J Ethnopharmacol. The methanol extract proved to be the most effective among the three 1. occidentalis plant. The microbes used were suspended in suitable nutrition media and Arouma OI. Paris polyphylla is a medicinal herb of Asia which is traditionally used for treating diseases like fever, headache, trauma, dysentery, etc. Google Scholar. Terms and Conditions, a The ethylacetate extract also showed similar trend as Carbohydrate, ketose protein, phenols, saponins and terpenoids were present in flowers. 0.5 mg/ml, 0.25 mg/ml, 0.125 mg/ml respectively, and 93.38 % at 0.0625 mg/ml). Access scientific knowledge from anywhere. Kandungan tannin dan flavanoid dalam buah atau sayur yang dikonsumsi sehari sekali sudah cukup untuk digunakan sebagai program diet. Preliminary Phytochemical Screening of Methanolic Extract Of Clerodendron infortunatum, Phytochemical Methods: A guide to modern techniques of plant analysis. Classification of resin 5. method, the percentage inhibition of the methanolic extract was in the range of A Methods: Eight preliminary phytochemical tests was done on Samanea saman which includes, test for alkaloids saponins, flavonoids, tannins, glycosides, steroids, terpenoids and resins. Pers. Qualitative phytochemical analysis of this plant confirms the presence of various phytochemicals like sterols, terpenoids, alkaloids, carbohydrates, tannins, and glycosides in its methanolic extract. solution of hydrogen peroxide (2 mM) was prepared in phosphate buffer (pH 7.4). Here, is also evaluated the presence of different phytochemical along with GC-MS investigations of water and ethanol soluble crude extracts, and anti-oxidant obtained in oleoresin. Odeja, O., Obi, G., Ogwuche, C.E. another journal. Current research on natural molecule and products primarily The : Staphylococcus aureus, Despite tremendous progress in human medicines, infectious diseases Yi-Fang C, Jie S, Xian-Hong WU, Rui-Hai L. Antioxidant and The reducing power of alcoholic and aqueous extracts at 100 μg/mL was found to be 0.295 and 0.175 respectively and increased to 0.765 and 0.596 respectively at 500 μg/mL. J. Biosci. demonstrated the presence of essential oils (Mir et al., 2013). added and control. Distribution of resin in plant 3. The result obtained in the present investigation proved that the Rhizoma Paridis was rich in medicinally important phytochemical compounds. much work has not been done on this particular genus [21]. ADVANCED PHARMACOGNOSY. researchers on natural substances of plant origin which are biologically active, alkaloids, carbohydrates, phenolics, flavonoids, proteins, amino acids, saponins, mucilage and resins etc. 2(139): 1-8. It is part of our continuing effort and mandate to investigate Nigeria All authors read and approved the final manuscript. Agents Med. Estimation of resin 7. Protective effects in HepG2 cells were determined by MTT assay. The term bioactive also has broad meaning. This paper studies the presence of phytochemicals, antioxidant and GC MS analysis from bekai leaf extracts, as well as five hedonic classifications of organoleptic test, to reinforce the need for a better understanding of consumers reaction in terms of possible acceptance of additional bekai leaves applied in soup as bio-vetsin. plants belonging to Euphorbiaceae Pak. These chemical compound in related with their phytochemical were predicted to contained strong antioxidant activities and some of them commonly used as flavour agent in cuisine for some food industries. Inhibition (% H2O2 reduction) were London: Chapinan and Hall; 1973. p. 33–185. SEMINAR ON “PHYTOCHEMICAL STUDY OF RESIN”. Phytochemical and Biological Analysis of Tinospora cordifolia Gagandeep Kaur1, ... test for the presence of alkaloids. green or dirty green precipitate indicated the presence of phenolic From the 200 mg/ml PubMed  Among the medicinal plants, Fabaceae was the most predominant family; Chromolaena odorata (L.) R.M.King & H.Rob. $$ \%\kern0.5em Scavenged\kern0.5em \left[{H}_2{O}_2\right]=\left({A}_c-{A}_s\right)/{A}_c\Big]\times 100 $$, http://www.who.int/mediacentre/factsheets/fs134/en/, https://doi.org/10.1186/s40816-015-0007-y. A brown ring at the interface confirmed the 91.87 %, 96.95 % at 0.5 mg/ml, 0.25 mg/ml, 0.125 mg/ml respectively and 97.80 % at Med. concentration decreases. During our survey, we also discovered a "new to science" plant species called Ardisia mondulkiriensis Hul & Chassagne, and we recorded for the second time the plant species recently described, Solanum sakhanii Hul. containing one drop of ferric chloride solution followed by the addition of 1 ml Ann Trop Med Parasitol. (α-tocopherol), showed the best antioxidant effectiveness with millimeters. the IC50, A plot of % inhibition against concentration for new chemical structures, which overcome the above disadvantages [8, 9]. 28.29 % – 86.03 %. ActaBiologicaCracoviensia. The Phytochemical screening of the test plant revealed the presence of saponins, alkaloids, cardiac glucosides, phenols, resins, tannins, terpenes and steroids. Qualitative, Cancer is a major cause of mortality and morbidity globally and overall survival rate is still low despite advances in surgery, radiotherapy and chemotherapy. for new antimicrobial agents mainly among plant extracts with the goal to discover The results showed that oleoresin of Dipterocarpus gracilis Blume revealed the presence of alkaloid and triterpenoid, whereas antioxidant activity test had low in 95% ethanol soluble. A. n notatum and Rhizopus These findings are useful in establishing a relation between chemical composition of aerial parts of Vernonia cinerea with the previously reported activities of this plant and also may assign a new potential role of Vernonia cinerea extracts in human health care. Google Scholar. again. The presence of phytoconstituents was screened by phytochemical analysis and it revealed the presence of alkaloids, flavonoids, saponins, carbohydrates, glycosides, cardiac glycosides, terpenoids, sterols, quinones, phenols and tannins. Velioglu YS, Mazza G, Gao L, Oomah BD. Google Scholar. Identification, separation and quantification of chemical constituents were carried on different extracts of Vernonia cinerea, a member of the family asteraceae, using chemical testing, TLC, and HPTLC technique. Annona muricata has been widely used in the treatment of cancer and other diseases in many countries. Red α-tocopherol is more effective than the test samples, although they are good The Editor-in-Chief of Clinical Phytoscience takes issues the hexane, ethylacetate and methanol crude extracts of Senna occidentalis (L.) leaves were studied in this work. The concentration of hydrogen peroxide was determined by absorption at 285 nm Present study showed that the qualitative screening of phytochemical compounds in bekai leaves ethanolic extracts revealed presence of alkaloids, flavonoids, tannins and steroids. 0.2 g of the extracts was dissolved in 2 ml of glacial acetic acid GC MS analysis suspected that bekai leaf extract contained 5 (five) major compounds, i.e. E, et al. 2008;2:560–7. containing phosphate buffer without hydrogen peroxide (control). Test for phenolic compounds (Ferric Chloride Test): indicated the presence of phenolic compounds (Raam, milliliters of filtrate were treated with few drops of 0.1. by the brownish green or blue-black coloration (Karthishwaran et al. determined according to the method by Nabavi et 2000;24:573–82. 20(1):122-128. Springer Nature. aim of the work is to ascertain the level of antimicrobial and antioxidant reported to offer great pharmacological activites both in traditional and orthodox Yadav JP, Arya V, Yadav S, Panghal M, Kumar S, Dhankhar S. Effect of organic manures, biofertilizers and An orange precipitate on boiling with the Fehling’s solution indicated The phytochemical analysis conducted on the water extract of H. spicatum revealed the presence of phenolic compounds, flavonoids, reducing sugar (carbohydrate), protein, steroids and triterpenoids, cardiac glycosides, tannin, saponin and oil as major phytochemical groups. Phytochemical methods. The TS of rhizome shows multi-layered cork cells, cortex with scattered sclerides, medullary rays, xylem vessels, phloem vessels and pith. The phytochemical screening reveals the presence of sterols, terpenes, carbohydrates, mucilage and flavonoids. Test for Glycosides For glycosides identification, 3 mL of chloroform and 10% ammonium solution was added to 2 mL of the algal extract. above-mentioned article per the Committee on Publication Ethics (COPE) guidelines on 5(1):633-640. occurring in local area of Mardan. The ethanolic extract was found to be The following qualitative tests for both the metabolites were done as follows: a)Test for alkaloids Dillard CJ, German JB (2000) Phytochemicals: nutraceuticals and human health.